Handheld FRET-Aptamer Sensor for Bone Markers, Phase I

Astronauts lose approximately 1-1.5% of their bone mass per month during space travel due to a lack of physical stress in the microgravity environment. Although, no effective treatments or prophylactic regimens have yet been defined, it is important to monitor the bone loss process in space. As such, the sensor must be compact and facile to operate. Therefore, OpTech proposes to extend its already successful and patent-pending competitive fluorescence resonance energy transfer (FRET)-aptamer assay technology to the detection of bone loss and formation markers such as osteocalcin fragments, hydroxylysine, hydroxyproline, C-terminal and N-terminal telopeptides. In Phase I, OpTech will develop, clone and sequence aptamers to each of these markers. OpTech will also incorporate fluorophore-labeled dUTP into the sequenced aptamers by asymmetric PCR and complex them to their quencher-labeled bone markers for testing in buffer, animal sera, and urine. Finally, in Phase I OpTech will dry and reconstitute the assays that will be tested using a commercially available handheld, battery-operated fluorometer and validated using OpTech's spectrofluorometer. In Phase II, the FRET-aptamer assays will be optimized and packaged in special leak-proof sealed plastic cuvettes and delivered to NASA along with the handheld fluorometer for testing on the ISS or other space missions.

This is a mature technology (TRL 7+) — the realistic path in is usually NASA's Technology Transfer Program: licensing an existing NASA patent, or a Space Act Agreement to use NASA facilities/expertise directly. NASA also runs a startup licensing program with no upfront fee for companies formed to commercialize a specific NASA technology.

None of these are guaranteed paths for this specific project — TechPort itself doesn't have an "apply" button. Reaching out to the contact(s) above with a specific question is usually the fastest way to find out what's actually open.